During the Reformation in the 16th century, the Danish crown shut down the convent and seized its assets. But even today there are many relics from the time of the nuns at Bosjökloster, among them the original vaults (once the nuns' refectory), the church, which still holds services, ruins of the penitential chapel, the little churchyard where the nuns lie buried under simple stones, and the thousand-year old oak, which still comes into leaf every year in the park.

The first private owner of Bosjökloster was a women, Mrs. Thale Ulfstand. She acquired the former convent and its remaining land through a barter transaction with the Danish King Fredrik II in 1560. A few decades later, in the 17th century, the property was taken over by the rich Jochum Beck, a versatile man and enthusiastic dilettante in many sciences. He lost his money through a failed industrial venture and was forced to sell Bosjökloster to pay his debts. The buyer Corfitz Ulfeldt was related to Beck. He fared no better. In this period, as Sweden was in the process of annexing Skåne from Denmark, Ulfeldt so lacked diplomacy in his intrigues with both the Swedish and Danish kings that he had to flee, and his wife Leonora Christina — the Danish king's favourite daughter — was imprisoned in the Blue Tower in Copenhagen for nearly 22 years.